Recombinant human USE1 protein
USE1, as known as P31, is a vesicle transport protein. This protein is a component of a SNARE complex consisting of STX18, uSE1L, BNIP1/SEC20L and SEC22B. It interacts directly with STX18. SNARE may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Recombinant human uSE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
SNARE-like tail-anchored protein 1 homolog, USE1-like protein (USE1L), unconventional SNARE in the ER 1 homolog, p31, SLT1, MDS032, D12, USE1L
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
28.3 kDa (254aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Meiringer CT. et al. (2011) J Biol Chem. 286:25039-25046; ; Dilcher M. et al. (2003) EMBO J. 22 (14) :3664-74.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl, 1mM DTT
Scientific Category
Stem cell
NCBI Accession Number
NP_060937
Uniprot Accession Number
Q9NZ43
Species
Human
AA Sequence
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