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Recombinant human USE1 protein

USE1, as known as P31, is a vesicle transport protein. This protein is a component of a SNARE complex consisting of STX18, uSE1L, BNIP1/SEC20L and SEC22B. It interacts directly with STX18. SNARE may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Recombinant human uSE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

SNARE-like tail-anchored protein 1 homolog, USE1-like protein (USE1L), unconventional SNARE in the ER 1 homolog, p31, SLT1, MDS032, D12, USE1L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

28.3 kDa (254aa) confirmed by MALDI-TOF

Additionnal Information

USE1, Vesicle transport protein uSE1, MDS032, P31, SLT1, ATGP2099-10 µg, ATGP2099-20 µg, ATGP2099-50 µg, ATGP2099-100 µg, ATGP2099-250 µg, ATGP2099-500 µg, ATGP2099-1 mg, ATGP2099-10, ATGP2099-20, ATGP2099-50, ATGP2099-100, ATGP2099-250, ATGP2099-500, ATGP2099-1

References & Citations

Meiringer CT. et al. (2011) J Biol Chem. 286:25039-25046; ; Dilcher M. et al. (2003) EMBO J. 22 (14) :3664-74.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl, 1mM DTT

Scientific Category

Stem cell

NCBI Accession Number

NP_060937

Uniprot Accession Number

Q9NZ43

Species

Human

AA Sequence

MAASRLE LNLVRLLSRC EAMAAEKRDP DEWRLEKYVG ALEDMLQALK VHASKPASEV INEYSWKVDF LKGMLQAEKL TSSSEKALAN QFLAPGRVPT TARERVPATK TVHLQSRARY TSEMRSELLG TDSAEPEMDV RKRTGVAGSQ PVSEKQSAAE LDLVLQRHQN LQEKLAEEML GLARSLKTNT LAAQSVIKKD NQTLSHSLKM ADQNLEKLKT ESERLEQHTQ KSVN

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