Recombinant human U2AF1 protein

U2AF1 belongs to the splicing factor SR family of genes. u2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of u2 snRNP to the pre-mRNA branch site. This protein is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Recombinant human u2AF1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

U2 small nuclear RNA auxiliary factor 1, FP793, RN, RNu2AF1, Splicing factor u2AF 35 kDa subunit, u2 auxiliary factor 35 kDa subunit, RN, RNu2AF1, u2AF35, u2AFBP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.3 kDa (263aa)

Additionnal Information

U2AF1, u2 small nuclear RNA auxiliary factor 1, FP793, RN, RNu2AF1, Splicing factor u2AF 35 kDa subunit, u2 auxiliary factor 35 kDa subunit, RN, RNu2AF1, u2AF35, u2AFBP, ATGP2095-10 µg, ATGP2095-20 µg, ATGP2095-50 µg, ATGP2095-100 µg, ATGP2095-250 µg, ATGP2095-500 µg, ATGP2095-1 mg, ATGP2095-10, ATGP2095-20, ATGP2095-50, ATGP2095-100, ATGP2095-250, ATGP2095-500, ATGP2095-1

References & Citations

Thol, F., et al. (2012) Blood 119 (15), 3578-3584; ; Makishima, H., et al. (2012) Blood 119 (14), 3203-3210

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001020374

Uniprot Accession Number

Q01081

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF