Recombinant human Arylsulfatase A/ARSA protein

ARSA hydrolyzes cerebrosidesulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. Recombinant human ARSA protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

ASA, Cerebroside-sulfatase, metachromatic leucodystrophy (MLD)

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

54.3 kDa (512aa)

Additionnal Information

ARSA, Arylsulfatase A, MLD, ATGP2087-10 µg, ATGP2087-20 µg, ATGP2087-50 µg, ATGP2087-100 µg, ATGP2087-250 µg, ATGP2087-500 µg, ATGP2087-1 mg, ATGP2087-10, ATGP2087-20, ATGP2087-50, ATGP2087-100, ATGP2087-250, ATGP2087-500, ATGP2087-1

References & Citations

Matthes, F., et al. (2011) J. Biol. Chem. 286 (20), 17487-17494; ; Hayashi, T., et al. (2011) Psychiatry Clin. Neurosci. 65 (1), 105-108

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000478.3

Uniprot Accession Number

P15289

Species

Human

AA Sequence

RPPNIVL IFADDLGYGD LGCYGHPSST TPNLDQLAAG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRM GMYPGVLVPS SRGGLPLEEV TVAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPA TPCDGGCDQG LVPIPLLANL SVEAQPPWLP GLEARYMAFA HDLMADAQRQ DRPFFLYYAS HHTHYPQFSG QSFAERSGRG PFGDSLMELD AAVGTLMTAI GDLGLLEETL VIFTADNGPE TMRMSRGGCS GLLRCGKGTT YEGGVREPAL AFWPGHIAPG VTHELASSLD LLPTLAALAG APLPNVTLDG FDLSPLLLGT GKSPRQSLFF YPSYPDEVRG VFAVRTGKYK AHFFTQGSAH SDTTADPACH ASSSLTAHEP PLLYDLSKDP GENYNLLGGV AGATPEVLQA LKQLQLLKAQ LDAAVTFGPS QVARGEDPAL QICCHPGCTP RPACCHCPDP HA