Recombinant human Histone H3.3/H3-3A protein

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A is a replication-independent member of the histone H3 family. Recombinant human H3F3A protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Histone H3.3, H3.3A, H3F3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

17.7 kDa (159aa)

Additionnal Information

H3F3A, Histone H3.3, H3.3A, H3F3, ATGP2083-10 µg, ATGP2083-20 µg, ATGP2083-50 µg, ATGP2083-100 µg, ATGP2083-250 µg, ATGP2083-500 µg, ATGP2083-1 mg, ATGP2083-10, ATGP2083-20, ATGP2083-50, ATGP2083-100, ATGP2083-250, ATGP2083-500, ATGP2083-1

References & Citations

Tagami H., et al. (2004) Cell. 116:51-61; ; Daury L N., et al. (2006) EMBO Rep. 7:66-71

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002098

Uniprot Accession Number

P84243

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA