Recombinant human ZFAND1 protein
AN1-type zinc finger protein 1 isoform a, also known as ZFAND1, belongs to the Zinc-finger proteins. Zinc -finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZFAND1 is a 268 amino acid protein that contains 2 AN1-type zinc fingers, which are often found in proteins that contain an ubiquitin-like domain and therefore play a role in the ubiquitination pathway. ZFAND1 contains six conserved cysteines and two histidines and have a dimetal (zinc) -bound alpha/beta fold. There are two isoforms of ZFAND1 that are produced as a result of alternative splicing events. Recombinant human ZFAND1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
AN1-type zinc finger protein 1 isoform a, AN1-type zinc finger protein 1 isoform a, zinc finger, AN1-type domain
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
33.2 kDa (291aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Laity J H., et al. (2001) Curr Opin Struct Biol. 11:39-46; ; Matthews J M., et al. (2002) IuBMB Life. 54:351-355.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Other
NCBI Accession Number
NP_078975
Uniprot Accession Number
Q8TCF1
Species
Human
AA Sequence
Frequently Asked Questions
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