Recombinant human PDZK1 protein

PDZK1 is a PDZ domain-containing scaffolding protein. This protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Recombinant human PDZK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Na (+) /H (+) exchange regulatory cofactor NHE-RF3, Na (+) /H (+) exchange regulatory cofactor NHE-RF3, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

59 kDa (542aa)

Additionnal Information

PDZK1, Na (+) /H (+) exchange regulatory cofactor NHE-RF3, Na (+) /H (+) exchange regulatory cofactor NHE-RF3, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1, ATGP2075-10 µg, ATGP2075-20 µg, ATGP2075-50 µg, ATGP2075-100 µg, ATGP2075-250 µg, ATGP2075-500 µg, ATGP2075-1 mg, ATGP2075-10, ATGP2075-20, ATGP2075-50, ATGP2075-100, ATGP2075-250, ATGP2075-500, ATGP2075-1

References & Citations

Kocher O, Yesilaltay A, et al. (2003) . J Biol Chem. 278 (52) :52820-5.; ; Inoue J, Otsuki T, et al. (2004) . Am J Pathol. 165 (1) :71-81.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_001188254

Uniprot Accession Number

Q5T2W1

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTSTFNP RECKLSKQEG QNYGFFLRIE KDTEGHLVRV VEKCSPAEKA GLQDGDRVLR INGVFVDKEE HMQVVDLVRK SGNSVTLLVL DGDSYEKAVK TRVDLKELGQ SQKEQGLSDN ILSPVMNGGV QTWTQPRLCY LVKEGGSYGF SLKTVQGKKG VYMTDITPQG VAMRAGVLAD DHLIEVNGEN VEDASHEEVV EKVKKSGSRV MFLLVDKETD KRHVEQKIQF KRETASLKLL PHQPRIVEMK KGSNGYGFYL RAGSEQKGQI IKDIDSGSPA EEAGLKNNDL VVAVNGESVE TLDHDSVVEM IRKGGDQTSL LVVDKETDNM YRLAHFSPFL YYQSQELPNG SVKEAPAPTP TSLEVSSPPD TTEEVDHKPK LCRLAKGENG YGFHLNAIRG LPGSFIKEVQ KGGPADLAGL EDEDVIIEVN GVNVLDEPYE KVVDRIQSSG KNVTLLVCGK KAYDYFQAKK IPIVSSLADP LDTPPDSKEG IVVESNHDSH MAKERAHSTA SHSSSNSEDT EM