Recombinant human DKK1 protein

Dickkopf-related protein 1, also known as DKK1, antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Recombinant human DKK1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Dickkopf WNT signaling pathway inhibitor 1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, SK

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.2 kDa (258aa)

Additionnal Information

Dickkopf 1, Dickkopf 1 homolog, Dickkopf 1 like, Dickkopf homolog 1, Dickkopf related protein 1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf-related protein 1 Dickkopf 1, DKK 1, DKK1, DKK-1, hDkk 1, hDkk1, hDkk-1, SK, Xenopus laevis, ATGP2070-10 µg, ATGP2070-20 µg, ATGP2070-50 µg, ATGP2070-100 µg, ATGP2070-250 µg, ATGP2070-500 µg, ATGP2070-1 mg, ATGP2070-010, ATGP2070-020, ATGP2070-050, ATGP2070-100, ATGP2070-250, ATGP2070-500, ATGP2070-01M

References & Citations

Ahn V.E., et al. (2011) Dev. Cell. 21:862-873; ; Haniu M., et al. (2011) Protein Sci. 20:1802-1813;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Development

NCBI Accession Number

NP_036374

Uniprot Accession Number

O94907

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRH