Recombinant human DKK1 protein
Dickkopf-related protein 1, also known as DKK1, antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Recombinant human DKK1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Dickkopf WNT signaling pathway inhibitor 1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, SK
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
28.2 kDa (258aa)
Additionnal Information
References & Citations
Ahn V.E., et al. (2011) Dev. Cell. 21:862-873; ; Haniu M., et al. (2011) Protein Sci. 20:1802-1813;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Development
NCBI Accession Number
NP_036374
Uniprot Accession Number
O94907
Species
Human
AA Sequence
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