Recombinant human CYB5R1 protein
Cytochrome b5 reductase 1, also known as CYB5R1, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. This protein is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Recombinant human CYB5R1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cytochrome b5 reductase 1, B5R.1, B5R1, B5R2, humb5R2, NQO3A2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
33.8 kDa (300aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zhu H., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:14742-14747; ; Clark H.F., et al. (2003) Genome Res. 13:2265-2270;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_057327
Uniprot Accession Number
Q9UHQ9
Species
Human
AA Sequence
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