Recombinant human CYB5R1 protein

Cytochrome b5 reductase 1, also known as CYB5R1, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. This protein is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Recombinant human CYB5R1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cytochrome b5 reductase 1, B5R.1, B5R1, B5R2, humb5R2, NQO3A2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

33.8 kDa (300aa) confirmed by MALDI-TOF

Additionnal Information

CYB5R1, Cytochrome b5 reductase 1, B5R.1, B5R1, B5R2, humb5R2, NQO3A2, ATGP2066-10 µg, ATGP2066-20 µg, ATGP2066-50 µg, ATGP2066-100 µg, ATGP2066-250 µg, ATGP2066-500 µg, ATGP2066-1 mg, ATGP2066-10, ATGP2066-20, ATGP2066-50, ATGP2066-100, ATGP2066-250, ATGP2066-500, ATGP2066-1

References & Citations

Zhu H., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:14742-14747; ; Clark H.F., et al. (2003) Genome Res. 13:2265-2270;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_057327

Uniprot Accession Number

Q9UHQ9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSLVRRSRR PQVTLLDPNE KYLLRLLDKT TVSHNTKRFR FALPTAHHTL GLPVGKHIYL STRIDGSLVI RPYTPVTSDE DQGYVDLVIK VYLKGVHPKF PEGGKMSQYL DSLKVGDVVE FRGPSGLLTY TGKGHFNIQP NKKSPPEPRV AKKLGMIAGG TGITPMLQLI RAILKVPEDP TQCFLLFANQ TEKDIILRED LEELQARYPN RFKLWFTLDH PPKDWAYSKG FVTADMIREH LPAPGDDVLV LLCGPPPMVQ LACHPNLDKL GYSQKMRFTY