Recombinant human MafF protein

MAFF is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the uS-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. This protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. Recombinant human MAFF protein, fused to His-tag at N-terminus, was expressed in E. coli

Product Specifications

Product Name Alternative

Transcription factor MafF isoform a, hMafF, u-MAF

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

20.1 kDa (187aa)

Additionnal Information

MafF, Transcription factor MafF isoform a, hMafF, u-MAF, ATGP2065-10 µg, ATGP2065-20 µg, ATGP2065-50 µg, ATGP2065-100 µg, ATGP2065-250 µg, ATGP2065-500 µg, ATGP2065-1 mg, ATGP2065-10, ATGP2065-20, ATGP2065-50, ATGP2065-100, ATGP2065-250, ATGP2065-500, ATGP2065-1

References & Citations

Ye X. et al. (2006) Arch. Biochem. Biophys. 449:87-93; ; Massrieh W. et al. (2006) Biol. Reprod. 74:699-705.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_001155045

Uniprot Accession Number

Q9ULX9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSVDPLS SKALKIKREL SENTPHLSDE ALMGLSVREL NRHLRGLSAE EVTRLKQRRR TLKNRGYAAS CRVKRVCQKE ELQKQKSELE REVDKLAREN AAMRLELDAL RGKCEALQGF ARSVAAARGP ATLVAPASVI TIVKSTPGSG SGPAHGPDPA HGPASCS