Recombinant human K-Ras protein

KRAS is a member of the small GTPase superfamily. This protein is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. under normal conditions, Ras family members influence cell growth and differentiation events in a subcellular membrane compartmentalization-based signaling system. Oncogenic Ras can deregulate processes that control both cell proliferation and apoptosis. Recombinant human KRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

GTPase Kras, KRAS proto-oncogene GTPase, KRAS2, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, Kirsten rat sarcoma viral oncogene homolog, KRAS1, K-Ras4B, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, RASK2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.8 kDa (210aa) confirmed by MALDI-TOF

Additionnal Information

KRAS, GTPase KRas isoform a precursor, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, ATGP2062-10 µg, ATGP2062-20 µg, ATGP2062-50 µg, ATGP2062-100 µg, ATGP2062-250 µg, ATGP2062-500 µg, ATGP2062-1 mg, ATGP2062-10, ATGP2062-20, ATGP2062-50, ATGP2062-100, ATGP2062-250, ATGP2062-500, ATGP2062-1

References & Citations

Lee W. et al. (2010) Nature. 465:473-477.; ; Singh M. et al. (2010) Nat Biotechnol. 28:585-593.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_203524.1

Uniprot Accession Number

P01116

Species

Human

AA Sequence

MTEYKL VVVGAGGVGK SALTIQLIQN HFVDEYDPTI EDSYRKQVVI DGETCLLDIL DTAGQEEYSA MRDQYMRTGE GFLCVFAINN TKSFEDIHHY REQIKRVKDS EDVPMVLVGN KCDLPSRTVD TKQAQDLARS YGIPFIETSA KTRQRVEDAF YTLVREIRQY RLKKISKEEK TPGCVKIKKC