Recombinant human GIP protein
GIP, also known as glucose-dependent insulinotropic polypeptide, is an important insulin-releasing hormone of the enteroinsular axis that has a functional profile of possible therapeutic value for type 2 diabetes. This protein is an important incretin hormone released into the circulation from endocrine K-cells of the duodenum and jejunum after ingestion of food1. It was evaluated for their ability to elevate cellular cAMP production and stimulate insulin secretion. It also promotes plasma triglyceride clearance in response to oral fat loading. In liver, GIP has been shown to enhance insulin-dependent inhibition of glycogenolysis. Recombinant human GIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Gastric inhibitory polypeptide, Glucose-dependent insulinotropic polypeptide, Gastric Inhibitory Peptide
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
17.3 kDa (155aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
O'Harte FP. et al. (1999) Diabetes. 48:758-765; ; Gault VA. et al. (2002) Biosci Rep. 22:523-528.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol 0.1M NaCl, 2mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
AAH69100
Uniprot Accession Number
P09681
Species
Human
AA Sequence
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