Recombinant human GIP protein

GIP, also known as glucose-dependent insulinotropic polypeptide, is an important insulin-releasing hormone of the enteroinsular axis that has a functional profile of possible therapeutic value for type 2 diabetes. This protein is an important incretin hormone released into the circulation from endocrine K-cells of the duodenum and jejunum after ingestion of food1. It was evaluated for their ability to elevate cellular cAMP production and stimulate insulin secretion. It also promotes plasma triglyceride clearance in response to oral fat loading. In liver, GIP has been shown to enhance insulin-dependent inhibition of glycogenolysis. Recombinant human GIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Gastric inhibitory polypeptide, Glucose-dependent insulinotropic polypeptide, Gastric Inhibitory Peptide

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

17.3 kDa (155aa) confirmed by MALDI-TOF

Additionnal Information

GIP, Gastric inhibitory polypeptide, Glucose-dependent insulinotropic polypeptide, Gastric Inhibitory Peptide, ATGP2061-10 µg, ATGP2061-20 µg, ATGP2061-50 µg, ATGP2061-100 µg, ATGP2061-250 µg, ATGP2061-500 µg, ATGP2061-1 mg, ATGP2061-10, ATGP2061-20, ATGP2061-50, ATGP2061-100, ATGP2061-250, ATGP2061-500, ATGP2061-1

References & Citations

O'Harte FP. et al. (1999) Diabetes. 48:758-765; ; Gault VA. et al. (2002) Biosci Rep. 22:523-528.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol 0.1M NaCl, 2mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

AAH69100

Uniprot Accession Number

P09681

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSEKKEGHF SALPSLPVGS HAKVSSPQPR GPRYAEGTFI SDYSIAMDKI HQQDFVNWLL AQKGKKNDWK HNITQREARA LELAGQANRK EEEAVEPQSS PAKNPSDEDL LRDLLIQELL ACLLDQTNLC RLRSR