Recombinant human EHF protein

ETS homologous factor isoform 2, also known as EHF, is a distinct member of the ESE subfamily of Ets transcription factors. Ets factors constitute one important class of transcriptional regulators that play critical roles in hema-topoiesis, angiogenesis, organogenesis, oncogenesis and specification of neuronal connectivity. It is exclusively expressed in a subset of epithelial cells, with highest expression detected in glandular epithelium of the prostate, pancreas, salivary gland and trachea. EHF transactivates the c-Met promoter via three high affinity binding sites, which suggests that EHF may contribute to branching morphogenesis. Recombinant human EHF protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

ETS homologous factor isoform 2, ESE3, ESE3B, ESEJ

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.3 kDa (323aa)

Additionnal Information

EHF, ETS homologous factor isoform 2, ESE3, ESE3B, ESEJ, ATGP2059-10 µg, ATGP2059-20 µg, ATGP2059-50 µg, ATGP2059-100 µg, ATGP2059-250 µg, ATGP2059-500 µg, ATGP2059-1 mg, ATGP2059-10, ATGP2059-20, ATGP2059-50, ATGP2059-100, ATGP2059-250, ATGP2059-500, ATGP2059-1

References & Citations

Lin J H., et al. (1998) Cell. 95:393-407.; ; Kas K., et al. (2000) J Biol Chem. 275:2986-2998.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_036285

Uniprot Accession Number

Q9NZC4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMILEGGG VMNLNPGNNL LHQPPAWTDS YSTCNVSSGF FGGQWHEIHP QYWTKYQVWE WLQHLLDTNQ LDANCIPFQE FDINGEHLCS MSLQEFTRAA GTAGQLLYSN LQHLKWNGQC SSDLFQSTHN VIVKTEQTEP SIMNTWKDEN YLYDTNYGST VDLLDSKTFC RAQISMTTTS HLPVAESPDM KKEQDPPAKC HTKKHNPRGT HLWEFIRDIL LNPDKNPGLI KWEDRSEGVF RFLKSEAVAQ LWGKKKNNSS MTYEKLSRAM RYYYKREILE RVDGRRLVYK FGKNARGWRE NEN