Recombinant e.coli mutY protein

Adenine DNA glycosylase, also known as mutY, is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. mutY is crucial for the avoidance of mutations resulting from oxidative DNA damage. Increasing levels of mutY in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. It is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span. Recombinant E. coil mutY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Adenine DNA glycosylase, ECK2956, JW2928, mica, mutB

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

41.5 kDa (373aa) confirmed by MALDI-TOF

Additionnal Information

MutY, Adenine DNA glycosylase, ECK2956, JW2928, mica, mutB, ATGP2056-10 µg, ATGP2056-20 µg, ATGP2056-50 µg, ATGP2056-100 µg, ATGP2056-250 µg, ATGP2056-500 µg, ATGP2056-1 mg, ATGP2056-10, ATGP2056-20, ATGP2056-50, ATGP2056-100, ATGP2056-250, ATGP2056-500, ATGP2056-1

References & Citations

Lee H M., et al. (2004) J Neurochem. 88:394-400; ; Tao H., et al. (2004) Carcinogenesis. 25:1859-1866.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_417436

Uniprot Accession Number

P17802

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMQASQFS AQVLDWYDKY GRKTLPWQID KTPYKVWLSE VMLQQTQVAT VIPYFERFMA RFPTVTDLAN APLDEVLHLW TGLGYYARAR NLHKAAQQVA TLHGGKFPET FEEVAALPGV GRSTAGAILS LSLGKHFPIL DGNVKRVLAR CYAVSGWPGK KEVENKLWSL SEQVTPAVGV ERFNQAMMDL GAMICTRSKP KCSLCPLQNG CIAAANNSWA LYPGKKPKQT LPERTGYFLL LQHEDEVLLA QRPPSGLWGG LYCFPQFADE ESLRQWLAQR QIAADNLTQL TAFRHTFSHF HLDIVPMWLP VSSFTGCMDE GNALWYNLAQ PPSVGLAAPV ERLLQQLRTG APV