Recombinant e.coli mutY protein
Adenine DNA glycosylase, also known as mutY, is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. mutY is crucial for the avoidance of mutations resulting from oxidative DNA damage. Increasing levels of mutY in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. It is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span. Recombinant E. coil mutY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Adenine DNA glycosylase, ECK2956, JW2928, mica, mutB
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
41.5 kDa (373aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lee H M., et al. (2004) J Neurochem. 88:394-400; ; Tao H., et al. (2004) Carcinogenesis. 25:1859-1866.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_417436
Uniprot Accession Number
P17802
Species
E.coli
AA Sequence
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