Recombinant human MRPL1 protein

39S ribosomal protein L1, mitochondrial precursor, also known as MRPL1, is a 338 amino acid mitochondrial protein that exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRPL1 exists as two isoforms produced by alternative splicing. Isoform one of MRPL1 is ubiquitously expressed while isoform two is specifically expressed in heart. Recombinant human MRPL1 proteinto His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

39S ribosomal protein L1 mitochondrial precursor, 39S ribosomal protein L1, mitochondrial precursor, BM022, L1MT, MRP-L1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

33.8 kDa (298aa) confirmed by MALDI-TOF

Additionnal Information

MRPL1, 39S ribosomal protein L1 mitochondrial precursor, 39S ribosomal protein L1, mitochondrial precursor, BM022, L1MT, MRP-L1, ATGP2052-10 µg, ATGP2052-20 µg, ATGP2052-50 µg, ATGP2052-100 µg, ATGP2052-250 µg, ATGP2052-500 µg, ATGP2052-1 mg, ATGP2052-10, ATGP2052-20, ATGP2052-50, ATGP2052-100, ATGP2052-250, ATGP2052-500, ATGP2052-1

References & Citations

Spirina O., et al. (2000) Gene. 261:229-234; ; Kenmochi N., et al. (2001) Genomics. 77:65-70.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_064621

Uniprot Accession Number

Q9BYD6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSKKTKKGA KEKTPDEKKD EIEKIKAYPY MEGEPEDDVY LKRLYPRQIY EVEKAVHLLK KFQILDFTSP KQSVYLDLTL DMALGKKKNV EPFTSVLSLP YPFASEINKV AVFTENASEV KIAEENGAAF AGGTSLIQKI WDDEIVADFY VAVPEIMPEL NRLRKKLNKK YPKLSRNSIG RDIPKMLELF KNGHEIKVDE ERENFLQTKI ATLDMSSDQI AANLQAVINE VCRHRPLNLG PFVVRAFLRS STSEGLLLKI DPLLPKEVKN EESEKEDA