Recombinant human MRPL1 protein
39S ribosomal protein L1, mitochondrial precursor, also known as MRPL1, is a 338 amino acid mitochondrial protein that exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRPL1 exists as two isoforms produced by alternative splicing. Isoform one of MRPL1 is ubiquitously expressed while isoform two is specifically expressed in heart. Recombinant human MRPL1 proteinto His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
39S ribosomal protein L1 mitochondrial precursor, 39S ribosomal protein L1, mitochondrial precursor, BM022, L1MT, MRP-L1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
33.8 kDa (298aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Spirina O., et al. (2000) Gene. 261:229-234; ; Kenmochi N., et al. (2001) Genomics. 77:65-70.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_064621
Uniprot Accession Number
Q9BYD6
Species
Human
AA Sequence
Frequently Asked Questions
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