Recombinant human TMEFF1/Tomoregulin-1 protein

TMEFF1, also known as tomoregulin-1, is a type I transmembrane glycoprotein. This protein contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain of TMEFF1 can be released as a soluble protein. TMEFF1 is predominantly expressed in brain, but is downregulated in brain neoplasms. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto. Recombinant human TMEFF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tomoregulin-1, C9orf2, CT120.1, H7365, TR-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.9 kDa (314aa) confirmed by MALDI-TOF

Additionnal Information

TMEFF1, Tomoregulin1, Tomoregulin-1, C9orf2, CT120.1, H7365, TR-1, ATGP2050-10 µg, ATGP2050-20 µg, ATGP2050-50 µg, ATGP2050-100 µg, ATGP2050-250 µg, ATGP2050-500 µg, ATGP2050-1 mg, ATGP2050-10, ATGP2050-20, ATGP2050-50, ATGP2050-100, ATGP2050-250, ATGP2050-500, ATGP2050-1

References & Citations

Gery S, Yin D, et al. (2003) . Oncogene. 22 (18) :2723-7.; ; Harms PW, et al. (2003) . Genes Dev. 17 (21) :2624-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_003683.2

Uniprot Accession Number

Q8IYR6

Species

Human

AA Sequence

SNQPPGG GGGSGGDCPG GKGKSINCSE LNVRESDVRV CDESSCKYGG VCKEDGDGLK CACQFQCHTN YIPVCGSNGD TYQNECFLRR AACKHQKEIT VIARGPCYSD NGSGSGEGEE EGSGAEVHRK HSKCGPCKYK AECDEDAENV GCVCNIDCSG YSFNPVCASD GSSYNNPCFV REASCIKQEQ IDIRHLGHCT DTDDTSLLGK KDDGLQYRPD VKDASDQRED VYIGNHMPCP ENLNGYCIHG KCEFIYSTQK ASCRCESGYT GQHCEKTDFS ILYVVPSRQK LTHV