Recombinant human ID1 protein

ID1 is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Recombinant human ID1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-binding protein inhibitor ID-1, bHLHb24, ID

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

18.5 kDa (178aa) confirmed by MALDI-TOF

Additionnal Information

ID1, DNA-binding protein inhibitor ID-1, bHLHb24, ID, ATGP2046-10 µg, ATGP2046-20 µg, ATGP2046-50 µg, ATGP2046-100 µg, ATGP2046-250 µg, ATGP2046-500 µg, ATGP2046-1 mg, ATGP2046-10, ATGP2046-20, ATGP2046-50, ATGP2046-100, ATGP2046-250, ATGP2046-500, ATGP2046-1

References & Citations

Deed R.W., et al. (1994) Biochim. Biophys. Acta. 1219:160-162; ; Hara E., et al. (1994) J. Biol. Chem. 269:2139-2145;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_002156

Uniprot Accession Number

P41134

Species

Human

AA Sequence

MKVASGS TATAAAGPSC ALKAGKTASG AGEVVRCLSE QSVAISRCAG GAGARLPALL DEQQVNVLLY DMNGCYSRLK ELVPTLPQNR KVSKVEILQH VIDYIRDLQL ELNSESEVGT PGGRGLPVRA PLSTLNGEIS ALTAEAACVP ADDRILCR