Recombinant human ID1 protein
ID1 is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Recombinant human ID1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA-binding protein inhibitor ID-1, bHLHb24, ID
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
18.5 kDa (178aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Deed R.W., et al. (1994) Biochim. Biophys. Acta. 1219:160-162; ; Hara E., et al. (1994) J. Biol. Chem. 269:2139-2145;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_002156
Uniprot Accession Number
P41134
Species
Human
AA Sequence
Frequently Asked Questions
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