Recombinant human SPA17 protein

SPA17 is a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. Recombinant human SPA17 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sperm surface protein Sp17, CT22, SP17, SP17-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.8 kDa (174aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

SPA17, Sperm surface protein Sp17, CT22, SP17, SP17-1, ATGP2045-10 µg, ATGP2045-20 µg, ATGP2045-50 µg, ATGP2045-100 µg, ATGP2045-250 µg, ATGP2045-500 µg, ATGP2045-1 mg, ATGP2045-10, ATGP2045-20, ATGP2045-50, ATGP2045-100, ATGP2045-250, ATGP2045-500, ATGP2045-1

References & Citations

Buchli R., et al. (2002) Biochim. Biophys. Acta. 1578:29-42; ; Li Z., et al. (2007) Int. J. Cancer. 121:1507-1511;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_059121

Uniprot Accession Number

Q15506

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSIPFSN THYRIPQGFG NLLEGLTREI LREQPDNIPA FAAAYFESLL EKREKTNFDP AEWGSKVEDR FYNNHAFEEQ EPPEKSDPKQ EESQISGKEE ETSVTILDSS EEDKEKEEVA AVKIQAAFRG HIAREEAKKM KTNSLQNEEK EENK