Recombinant e.coli phrB protein
Deoxyribodipyrimidine photolyase, also known as phrB, is involved in repair of uV radiation-induced DNA damage. This protein catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Recombinant E. coli phrB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Deoxyribodipyrimidine photolyase, ECK0697, JW0698, phrB
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
56.1 kDa (495aa)
Additionnal Information
References & Citations
Byrdin M., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:8676-8681; ; Weber S., et al. (2005) Biochim. Biophys. Acta. 1707:1-23;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_415236.1
Uniprot Accession Number
P00914
Species
E.coli
AA Sequence
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