Recombinant human LAGE3 protein

L antigen family member 3, also known as LAGE3, belongs to the CTAG family. Members of the LAGE/ESO gene family are clustered together on human chromosome Xq28 and have similar exon-intron structures. unlike the other family members, which are normally expressed only in testis and activated in a wide range of human tumors, LAGE3 is ubiquitously expressed in somatic tissues. This protein is also highly conserved in mouse and rat, suggesting that the encoded protein is functionally important. Recombinant human LAGE3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

L antigen family member 3, CVG5, DXS9879E, DXS9951E, ESO3, ITBA2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

17.2 kDa (166aa) confirmed by MALDI-TOF

Additionnal Information

LAGE3, L antigen family member 3, CVG5, DXS9879E, DXS9951E, ESO3, ITBA2, ATGP2043-10 µg, ATGP2043-20 µg, ATGP2043-50 µg, ATGP2043-100 µg, ATGP2043-250 µg, ATGP2043-500 µg, ATGP2043-1 mg, ATGP2043-10, ATGP2043-20, ATGP2043-50, ATGP2043-100, ATGP2043-250, ATGP2043-500, ATGP2043-1

References & Citations

Alpen B., et al. (2002) Gene. 297:141-149.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,50% glycerol, 2mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006005

Uniprot Accession Number

Q14657

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMRDADAD AGGGADGGDG RGGHSCRGGV DTAAAPAGGA PPAHAPGPGR DAASAARGSR MRPHIFTLSV PFPTPLEAEI AHGSLAPDAE PHQRVVGKDL TVSGRILVVR WKAEDCRLLR ISVINFLDQL SLVVRTMQRF GPPVSR