Recombinant human CPOX protein

Coproporphyrinogen-III oxidase, mitochondrial precursor, also known as CPOX, is a 454 amino acid mitochondrial enzyme that is localized to the inner membrane space of erythrocytes. It participates in the sixth step of heme biosynthesis by catalyzing the formation of protoporphyrinogen IX from coproporphyrinogen III. Mutations in human CPOX gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria. Recombinant human CPOX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Coproporphyrinogen-III oxidase mitochondrial precursor, Coproporphyrinogen-III oxidase, mitochondrial precursor, CPO, CPX, HCP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

41.6 kDa (367aa) confirmed by MALDI-TOF

Additionnal Information

CPOX, Coproporphyrinogen-III oxidase mitochondrial precursor, Coproporphyrinogen-III oxidase, mitochondrial precursor, CPO, CPX, HCP, ATGP2042-10 µg, ATGP2042-20 µg, ATGP2042-50 µg, ATGP2042-100 µg, ATGP2042-250 µg, ATGP2042-500 µg, ATGP2042-1 mg, ATGP2042-10, ATGP2042-20, ATGP2042-50, ATGP2042-100, ATGP2042-250, ATGP2042-500, ATGP2042-1

References & Citations

Martasek P., et al. (1994) Hum Mol Genet. 3:477-480; ; Lamoril J., et al. (1995) Hum Mol Genet. 4:275-278.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_000088

Uniprot Accession Number

P36551

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSTSLGRPE EEEDELAHRC SSFMAPPVTD LGELRRRPGD MKTKMELLIL ETQAQVCQAL AQVDGGANFS VDRWERKEGG GGISCVLQDG CVFEKAGVSI SVVHGNLSEE AAKQMRSRGK VLKTKDGKLP FCAMGVSSVI HPKNPHAPTI HFNYRYFEVE EADGNKQWWF GGGCDLTPTY LNQEDAVHFH RTLKEACDQH GPDLYPKFKK WCDDYFFIAH RGERRGIGGI FFDDLDSPSK EEVFRFVQSC ARAVVPSYIP LVKKHCDDSF TPQEKLWQQL RRGRYVEFNL LYDRGTKFGL FTPGSRIESI LMSLPLTARW EYMHSPSENS KEAEILEVLR HPRDWVR