Recombinant human MARCKSL1 protein

MARCKS-related protein, also known as MARCKSL1, is a member of MARCKS family of PKC substrate. It is widely used in the signal transduction studies as an indicator of PKC activation. Expressed in a variety of tissues with highest levels found in testis and uterus, MARCKSL1 participates in the coordination of membrane-cytoskeletal signaling events, including secretion, migration, phagocytosis and cell adhesion. Additionally, MARCKSL1 functions as a regulator of Integrin activation and is thought to regulate Integrin-dependent signal transduction pathways, especially those involved in macrophage spreading. Recombinant human MARCKSL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

MARCKS-related protein, F52, MACMARCKS, MLP, MLP1, MRP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by BCA assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.9 kDa (218aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

MARCKSL1, MARCKS-related protein, F52, MACMARCKS, MLP, MLP1, MRP, ATGP2041-10 µg, ATGP2041-20 µg, ATGP2041-50 µg, ATGP2041-100 µg, ATGP2041-250 µg, ATGP2041-500 µg, ATGP2041-1 mg, ATGP2041-10, ATGP2041-20, ATGP2041-50, ATGP2041-100, ATGP2041-250, ATGP2041-500, ATGP2041-1

References & Citations

Jin T., et al. (2001) J Biol Chem. 276:12879-12884; ; Murphy A., et al. (2003) Neurosci Lett. 347:9-12.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_075385

Uniprot Accession Number

P49006

Species

Human

AA Sequence

MGSQSSK APRGDVTAEE AAGASPAKAN GQENGHVKSN GDLSPKGEGE SPPVNGTDEA AGATGDAIEP APPSQGAEAK GEVPPKETPK KKKKFSFKKP FKLSGLSFKR NRKEGGGDSS ASSPTEEEQE QGEIGACSDE GTAQEGKAAA TPESQEPQAK GAEASAASEE EAGPQATEPS TPSGPESGPT PASAEQNE