Recombinant human Immunoglobulin J chain/IGJ protein

Immunoglobulin J chain, also known as IGJ, serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory component. Recombinant human IGJ protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Joining chain of multimeric IgA and IgM, Immunoglobulin J chain, IgJ chain, J Chain, IGCJ, JCH, IGJ, JCHAIN

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

18 kDa (160aa) confirmed by MALDI-TOF

Additionnal Information

IGJ, Immunoglobulin J chain, IGCJ, JCH, ATGP2039-10 µg, ATGP2039-20 µg, ATGP2039-50 µg, ATGP2039-100 µg, ATGP2039-250 µg, ATGP2039-500 µg, ATGP2039-1 mg, ATGP2039-10, ATGP2039-20, ATGP2039-50, ATGP2039-100, ATGP2039-250, ATGP2039-500, ATGP2039-1

References & Citations

Liu T., et al. (2005) J. Proteome Res. 4:2070-2080; ; Ramachandran P., et al. (2006) J. Proteome Res. 5:1493-1503;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_653247.1

Uniprot Accession Number

P01591

Species

Human

AA Sequence

QEDERIV LVDNKCKCAR ITSRIIRSSE DPNEDIVERN IRIIVPLNNR ENISDPTSPL RTRFVYHLSD LCKKCDPTEV ELDNQIVTAT QSNICDEDSA TETCYTYDRN KCYTAVVPLV YGGETKMVET ALTPDACYPD