Recombinant human MRM1 protein

Mitochondrial rRNA methyltransferase 1 homolog, also known as MRM1, probably methylates the ribose of guanosine G-2270 in the peptidyl transferase center of the mitochondrial large ribosomal RNA (21S) . Recombinant human MRM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mitochondrial rRNA methyltransferase 1 homolog, FLJ22578, Mitochondrial large ribosomal RNA ribose methylase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.8 kDa (356aa) confirmed by MALDI-TOF

Additionnal Information

MRM1, Mitochondrial rRNA methyltransferase 1 homolog, FLJ22578, Mitochondrial large ribosomal RNA ribose methylase, ATGP2038-10 µg, ATGP2038-20 µg, ATGP2038-50 µg, ATGP2038-100 µg, ATGP2038-250 µg, ATGP2038-500 µg, ATGP2038-1 mg, ATGP2038-10, ATGP2038-20, ATGP2038-50, ATGP2038-100, ATGP2038-250, ATGP2038-500, ATGP2038-1

References & Citations

Ota T., et al. (2004) Nat. Genet. 36:40-45

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT, 0.1mM PMSF

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_079140

Uniprot Accession Number

Q6IN84

Species

Human

AA Sequence

SHAARHG ERPGGEELSR LLLDDLVPTS RLELLFGMTP CLLALQAARR SVARLLLQAG KAGLQGKRAE LLRMAEARDI PVLRPRRQKL DTMCRYQVHQ GVCMEVSPLR PRPWREAGEA SPGDDPQQLW LVLDGIQDPR NFGAVLRSAH FLGVDKVITS RRNSCPLTPV VSKSSAGAME VMDVFSTDDL TGFLQTKAQQ GWLVAGTVGC PSTEDPQSSE IPIMSCLEFL WERPTLLVLG NEGSGLSQEV QASCQLLLTI LPRRQLPPGL ESLNVSVAAG ILLHSICSQR KGFPTEGERR QLLQDPQEPS ARSEGLSMAQ HPGLSSGPEK ERQNEG