Recombinant human CD46 protein

Membrane cofactor protein isoform 14, also known as CD46, is a type I membrane protein that has cofactor activity for inactivation of complement components C3b and C4b by serum factor I. This function resides in the complement control protein repeats (CCPs), with CCP1-4 essential for regulation. CD46 is widely distributed on thymocytes, T cells, B cells, monocytes, granulocytes, NK cells, platelets, endothelial cells, epithelial cells, fibroblasts, placenta and sperm, but not on erythrocyte. Recombinant human CD46 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Membrane cofactor protein isoform 14, AHuS2, MCP, MIC10, TLX, TRA2.10

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

33.8 kDa (302aa) confirmed by MALDI-TOF

Additionnal Information

CD46, Membrane cofactor protein isoform 14, AHuS2, MCP, MIC10, TLX, TRA2.10, ATGP2036-10 µg, ATGP2036-20 µg, ATGP2036-50 µg, ATGP2036-100 µg, ATGP2036-250 µg, ATGP2036-500 µg, ATGP2036-1 mg, ATGP2036-10, ATGP2036-20, ATGP2036-50, ATGP2036-100, ATGP2036-250, ATGP2036-500, ATGP2036-1

References & Citations

Riley R C., et al. (2002) Mol Reprod Dev. 62:534-546.; ; Iwata K., et al. (1995) J Biol Chem. 270:15148-15152.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_758860.1

Uniprot Accession Number

P15529

Species

Human

AA Sequence

CEEPPTF EAMELIGKPK PYYEIGERVD YKCKKGYFYI PPLATHTICD RNHTWLPVSD DACYRETCPY IRDPLNGQAV PANGTYEFGY QMHFICNEGY YLIGEEILYC ELKGSVAIWS GKPPICEKVL CTPPPKIKNG KHTFSEVEVF EYLDAVTYSC DPAPGPDPFS LIGESTIYCG DNSVWSRAAP ECKVVKCRFP VVENGKQISG FGKKFYYKAT VMFECDKGFY LDGSDTIVCD SNSTWDPPVP KCLKGPRPTY KPPVSNYPGY PKPEEGILDS LD