Recombinant human TDP-43/TARDBP protein

TARDBP was originally identified as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It has also been identified in individuals diagnosed with chronic traumatic encephalopathy, a condition that often mimics ALS and that has been associated with athletes who have experienced multiple concussions and other types of head injury. It has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recombinant human TARDBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

TAR DNA binding protein, ALS10, TDP43

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

33.6 kDa (296aa) confirmed by MALDI-TOF

Additionnal Information

TDP43, TARDBP, ATGP2032-10 µg, ATGP2032-20 µg, ATGP2032-50 µg, ATGP2032-100 µg, ATGP2032-250 µg, ATGP2032-500 µg, ATGP2032-1 mg, ATGP2032-10, ATGP2032-20, ATGP2032-50, ATGP2032-100, ATGP2032-250, ATGP2032-500, ATGP2032-1

References & Citations

Ou SH. et al. (1995) J Virol. 69:3584-3596.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_031401

Uniprot Accession Number

Q13148

Species

Human

AA Sequence

MSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNA