Recombinant human MOBKL1A/MOB1B protein

MOB1B is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human MOB1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

MOB kinase activator 1B isoform 2, MATS2, MOB4A, MOBKL1A, Mob1 homolog 1A, Mob1A, Mob1B, Mps one binder kinase activator-like 1A, MOB1 Mps One Binder homolog B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

27 kDa (239aa) confirmed by MALDI-TOF

Additionnal Information

MOB1B, MOB kinase activator 1B isoform 2, MATS2, MOB4A, MOBKL1A, ATGP2026-10 µg, ATGP2026-20 µg, ATGP2026-50 µg, ATGP2026-100 µg, ATGP2026-250 µg, ATGP2026-500 µg, ATGP2026-1 mg, ATGP2026-10, ATGP2026-20, ATGP2026-50, ATGP2026-100, ATGP2026-250, ATGP2026-500, ATGP2026-1

References & Citations

Chow A, Hao Y, et al. (2010) . Int J Cancer. 126 (9) :2079-89.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_775739

Uniprot Accession Number

Q7L9L4

Species

Human

AA Sequence

MSFLFGS RSSKTFKPKK NIPEGSHQYE LLKHAEATLG SGNLRMAVML PEGEDLNEWV AVNTVDFFNQ INMLYGTITD FCTEESCPVM SAGPKYEYHW ADGTNIKKPI KCSAPKYIDY LMTWVQDQLD DETLFPSKIG VPFPKNFMSV AKTILKRLFR VYAHIYHQHF DPVIQLQEEA HLNTSFKHFI FFVQEFNLID RRELAPLQEL IEKLTSKDR