Recombinant human TDP2 protein

TDP2 is a member of a superfamily of divalent cation-dependent phosphodiesterases. The protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. Recombinant human TDP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tyrosyl-DNA phosphodiesterase 2, AD022, dJ30M3.3, EAP2, EAPII, RP1-30M3.3, TTRAP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

43.3 kDa (385aa) confirmed by MALDI-TOF

Additionnal Information

TDP2, Tyrosyl-DNA phosphodiesterase 2, AD022, dJ30M3.3, EAP2, EAPII, RP1-30M3.3, TTRAP, ATGP2024-10 µg, ATGP2024-20 µg, ATGP2024-50 µg, ATGP2024-100 µg, ATGP2024-250 µg, ATGP2024-500 µg, ATGP2024-1 mg, ATGP2024-10, ATGP2024-20, ATGP2024-50, ATGP2024-100, ATGP2024-250, ATGP2024-500, ATGP2024-1

References & Citations

Vilotti, S., et al. (2012) Cell Death Differ. 19 (3), 488-500; ; Pinel, P., et al. (2012) J. Neurosci. 32 (3), 817-825;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_057698

Uniprot Accession Number

O95551

Species

Human

AA Sequence

MELGSCL EGGREAAEEE GEPEVKKRRL LCVEFASVAS CDAAVAQCFL AENDWEMERA LNSYFEPPVE ESALERRPET ISEPKTYVDL TNEETTDSTT SKISPSEDTQ QENGSMFSLI TWNIDGLDLN NLSERARGVC SYLALYSPDV IFLQEVIPPY YSYLKKRSSN YEIITGHEEG YFTAIMLKKS RVKLKSQEII PFPSTKMMRN LLCVHVNVSG NELCLMTSHL ESTRGHAAER MNQLKMVLKK MQEAPESATV IFAGDTNLRD REVTRCGGLP NNIVDVWEFL GKPKHCQYTW DTQMNSNLGI TAACKLRFDR IFFRAAAEEG HIIPRSLDLL GLEKLDCGRF PSDHWGLLCN LDIIL