Recombinant human CPSF4 protein

Inhibition of the nuclear export of poly (A) -containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of CPSF4 an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Recombinant human CPSF4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cleavage and polyadenylation specific factor 430kDa, cleavage and polyadenylation specific factor 4,30kDa, CPSF30, NAR, NEB1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.9 kDa (267aa)

Additionnal Information

CPSF4, cleavage and polyadenylation specific factor 430kDa, cleavage and polyadenylation specific factor 4,30kDa, CPSF30, NAR, NEB1, ATGP2023-10 µg, ATGP2023-20 µg, ATGP2023-50 µg, ATGP2023-100 µg, ATGP2023-250 µg, ATGP2023-500 µg, ATGP2023-1 mg, ATGP2023-10, ATGP2023-20, ATGP2023-50, ATGP2023-100, ATGP2023-250, ATGP2023-500, ATGP2023-1

References & Citations

Jenny, A., et al. (1994) Mol. Cell. Biol. 14 (12), 8183-8190; ; Barabino, S.M., et al. (1997) Nephrol Genes Dev. 11 (13), 1703-1716;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001075028

Uniprot Accession Number

O95639

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMQEIIAS VDHIKFDLEI AVEQQLGAQP LPFPGMDKSG AAVCEFFLKA ACGKGGMCPF RHISGEKTVV CKHWLRGLCK KGDQCEFLHE YDMTKMPECY FYSKFGECSN KECPFLHIDP ESKIKDCPWY DRGFCKHGPL CRHRHTRRVI CVNYLVGFCP EGPSCKFMHP RFELPMGTTE QPPLPQQTQP PAKQRTPQVI GVMQSQNSSA GNRGPRPLEQ VTCYKCGEKG HYANRCTKGH LAFLSGQ