Recombinant human ACAA2 protein

ACAA2 catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. Recombinant human ACAA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Acetyl-CoA acyltransferase 2, DSAEC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

42.6 kDa (404aa) confirmed by MALDI-TOF

Additionnal Information

ACAA, ATGP2020-10 µg, ATGP2020-20 µg, ATGP2020-50 µg, ATGP2020-100 µg, ATGP2020-250 µg, ATGP2020-500 µg, ATGP2020-1 mg, ATGP2020-10, ATGP2020-20, ATGP2020-50, ATGP2020-100, ATGP2020-250, ATGP2020-500, ATGP2020-1

References & Citations

Cao, W , et al. (2008) Biochim. Biophys. Acta 1780 (6), 873-880

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_006102

Uniprot Accession Number

P42765

Species

Human

AA Sequence

FGAYGGL LKDFTATDLS EFAAKAALSA GKVSPETVDS VIMGNVLQSS SDAIYLARHV GLRVGIPKET PALTINRLCG SGFQSIVNGC QEICVKEAEV VLCGGTESMS QAPYCVRNVR FGTKLGSDIK LEDSLWVSLT DQHVQLPMAM TAENLAVKHK ISREECDKYA LQSQQRWKAA NDAGYFNDEM APIEVKTKKG KQTMQVDEHA RPQTTLEQLQ KLPPVFKKDG TVTAGNASGV ADGAGAVIIA SEDAVKKHNF TPLARIVGYF VSGCDPSIMG IGPVPAISGA LKKAGLSLKD MDLVEVNEAF APQYLAVERS LDLDISKTNV NGGAIALGHP LGGSGSRITA HLVHELRRRG GKYAVGSACI GGGQGIAVII QSTA