Recombinant human YOD1 protein
YOD1 is a highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, whose function has yet to be assigned in mammalian cells. YOD1 is a constituent of a multiprotein complex with p97 as its nucleus, suggesting a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. Expression of a YOD1 variant deprived of its deubiquitinating activity imposes a halt on the dislocation reaction, as judged by the stabilization of various dislocation substrates. Recombinant human YOD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin thioesterase OTu1, ubiquitin thioesterase OTu1, DuBA8, OTuD2, PRO0907, RP11-164O23.1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
40.7 kDa (371aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ernst, R., et al. (2009) Mol. Cell 36 (1), 28-38
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_061036.3
Uniprot Accession Number
Q5VVQ6
Species
Human
AA Sequence
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