Recombinant human MRPL13 protein

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL13 is a 39S subunit protein. Recombinant human MRPL13 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Mitochondrial ribosomal protein L13, L13, L13A, L13mt, RPL13, RPML13

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

23.1 kDa (201aa)

Additionnal Information

MRPL13, Mitochondrial ribosomal protein L13, L13, L13A, L13mt, RPL13, RPML13, ATGP2018-10 µg, ATGP2018-20 µg, ATGP2018-50 µg, ATGP2018-100 µg, ATGP2018-250 µg, ATGP2018-500 µg, ATGP2018-1 mg, ATGP2018-10, ATGP2018-20, ATGP2018-50, ATGP2018-100, ATGP2018-250, ATGP2018-500, ATGP2018-1

References & Citations

Suzuki T., et al. (2001) J. Biol. Chem. 276, 21724-36.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_054797

Uniprot Accession Number

Q9BYD1

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSFSRA PQQWATFARI WYLLDGKMQP PGKLAAMASI RLQGLHKPVY HALSDCGDHV VIMNTRHIAF SGNKWEQKVY SSHTGYPGGF RQVTAAQLHL RDPVAIVKLA IYGMLPKNLH RRTMMERLHL FPDEYIPEDI LKNLVEELPQ PRKIPKRLDE YTQEEIDAFP RLWTPPEDYR L