Recombinant human Follistatin/FST protein

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Recombinant human FST protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Follistatin, Follistatin, FS, FST344

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37 kDa (336aa) confirmed by MALDI-TOF

Additionnal Information

FST, Follistatin, Follistatin, FS, ATGP2017-10 µg, ATGP2017-20 µg, ATGP2017-50 µg, ATGP2017-100 µg, ATGP2017-250 µg, ATGP2017-500 µg, ATGP2017-1 mg, ATGP2017-10, ATGP2017-20, ATGP2017-50, ATGP2017-100, ATGP2017-250, ATGP2017-500, ATGP2017-1

References & Citations

Lerch T.F., et al. (2007) J. Biol. Chem. 282:15930-15939; ; Cash J.N., et al. (2009) EMBO J. 28:2662-2676;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_037541

Uniprot Accession Number

P19883

Species

Human

AA Sequence

MGNCWLRQAK NGRCQVLYKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DFKVGRGRCS LCDELCPDSK SDEPVCASDN ATYASECAMK EAACSSGVLL EVKHSGSCNS ISEDTEEEEE DEDQDYSFPI SSILEW