Recombinant human GPR177/WLS protein

WLS, a Wnt trafficking regulator, is a multipass transmembrane protein that regulates the sorting and secretion of Wnt protein. It is required for organogenesis during embryogenesis and for the establishment of the anterior-posterior axis. WLS promotes various cancers such as glioma tumourigenesis. The gene encoding WLS maps to human chromosome 1 and is expressed as multiple alternatively spliced isoforms. Recombinant human WLS protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Protein wntless homolog isoform 1 precursor, C1orf139, EVI, GPR177, MRP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

17.8 kDa (155aa)

Additionnal Information

WLS, Protein wntless homolog isoform 1 precursor, C1orf139, EVI, GPR177, MRP, ATGP2015-10 µg, ATGP2015-20 µg, ATGP2015-50 µg, ATGP2015-100 µg, ATGP2015-250 µg, ATGP2015-500 µg, ATGP2015-1 mg, ATGP2015-10, ATGP2015-20, ATGP2015-50, ATGP2015-100, ATGP2015-250, ATGP2015-500, ATGP2015-1

References & Citations

Ji T H., et al. (1998) J Biol Chem. 273:17299-17302.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Stem cell

NCBI Accession Number

NP_079187

Uniprot Accession Number

Q5T9L3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEMSPWF QFMLFILQLD IAFKLNNQIR ENAEVSMDVS LAYRDDAFAE WTEMAHERVP RKLKCTFTSP KTPEHEGRYY ECDVLPFMEI GSVAHKFYLL NIRLPVNEKK KINVGIGEIK DIRLVGIHQN GGFTK