Recombinant human Serpin G1/SERPING1 protein

Plasma protease C1 inhibitor, also known as SERPING1, is a member of the serpin superfamily of serine protease inhibitors. SERPING1 regulates the activation of complement factor C1 as well as the activity of activated C1 by coupling with the active catalytic site at the light chains of C1r and C1s. Therefore, it plays an important role in regulating activation of both the complement and contact systems. SERPING1 deficiency results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa. Recombinant human SERPING1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Serpin family G member 1, C1NH, C1 Inh, C1 esterase inhibitor, C1-inhibiting factor, HAE1, HAE2, Plasma protease C1 inhibitor, C1-inhibitor

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

55.1 kDa (499aa)

Additionnal Information

SERPING1, SERPING 1, Serpin family G member 1, Plasma protease C1 inhibitor, HAE2, HAE1, HAE 2, HAE 1, C1NH, C1-inhibitor, C1-inhibiting factor, C1Inh, C1IN, C1 NH, C1 inhibitor, C1 inhibiting factor, C1 Inh, C1 esterase inhibitor, ATGP2014-10 µg, ATGP2014-20 µg, ATGP2014-50 µg, ATGP2014-100 µg, ATGP2014-250 µg, ATGP2014-500 µg, ATGP2014-1 mg, ATGP2014-010, ATGP2014-020, ATGP2014-050, ATGP2014-100, ATGP2014-250, ATGP2014-500, ATGP2014-01M

References & Citations

Davis A E., et al. (1993) Methods Enzymol. 223:97.; ; Davis A E., et al. (2004) Drog News Perspect. 17:439.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001027466

Uniprot Accession Number

P05155

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MNPNATSSSS QDPESLQDRG EGKVATTVIS KMLFVEPILE VSSLPTTNST TNSATKITAN TTDEPTTQPT TEPTTQPTIQ PTQPTTQLPT DSPTQPTTGS FCPGPVTLCS DLESHSTEAV LGDALVDFSL KLYHAFSAMK KVETNMAFSP FSIASLLTQV LLGAGENTKT NLESILSYPK DFTCVHQALK GFTTKGVTSV SQIFHSPDLA IRDTFVNASR TLYSSSPRVL SNNSDANLEL INTWVAKNTN NKISRLLDSL PSDTRLVLLN AIYLSAKWKT TFDPKKTRME PFHFKNSVIK VPMMNSKKYP VAHFIDQTLK AKVGQLQLSH NLSLVILVPQ NLKHRLEDME QALSPSVFKA IMEKLEMSKF QPTLLTLPRI KVTTSQDMLS IMEKLEFFDF SYDLNLCGLT EDPDLQVSAM QHQTVLELTE TGVEAAAASA ISVARTLLVF EVQQPFLFVL WDQQHKFPVF MGRVYDPRA