Recombinant human GULP1/CED-6 protein

PTB domain-containing engulfment adapter protein 1, also known as GuLP1, is an evolutionarily conserved adaptor protein required for efficient engulfment of apoptotic cells by phagocytes. GuLP1 also helps modulate cellular glycosphingolipid and cholesterol transport. It also may play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increased cytoplasmic levels of GuLP1 are associated with increases in cellular levels of GTP-bound ARF6. Recombinant human GuLP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

PTB domain-containing engulfment adapter protein 1, CED-6, GuLP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

36.9 kDa (327aa) confirmed by MALDI-TOF

Additionnal Information

PTB domain-containing engulfment adapter protein 1, PTB domain containing engulfment adapter protein 1, CED-6, CED6, GuLP, GULP1, CED 6, GULP 1, GULP-1, ATGP2009-10 µg, ATGP2009-20 µg, ATGP2009-50 µg, ATGP2009-100 µg, ATGP2009-250 µg, ATGP2009-500 µg, ATGP2009-1 mg, ATGP2009-010, ATGP2009-020, ATGP2009-050, ATGP2009-100, ATGP2009-250, ATGP2009-500, ATGP2009-01M

References & Citations

Smits E., et al. (2000) Curr Biol. 9:1351-1354; ; Su H P., et al. (2000) J Biol Chem. 275: 9542-9549.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057399

Uniprot Accession Number

Q9UBP9

Species

Human

AA Sequence

MNRAFSR KKDKTWMHTP EALSKHFIPY NAKFLGSTEV EQPKGTEVVR DAVRKLKFAR HIKKSEGQKI PKVELQISIY GVKILEPKTK EVQHNCQLHR ISFCADDKTD KRIFTFICKD SESNKHLCYV FDSEKCAEEI TLTIGQAFDL AYRKFLESGG KDVETRKQIA GLQKRIQDLE TENMELKNKV QDLENQLRIT QVSAPPAGSM TPKSPSTDIF DMIPFSPISH QSSMPTRNGT QPPPVPSRST EIKRDLFGAE PFDPFNCGAA DFPPDIQSKL DEMQEGFKMG LTLEGTVFCL DPLDSRC