Recombinant human CIAO1 protein

CIAO1 is essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. This protein is required for the maturation of extra mitochondrial Fe/S proteins and seems to specifically modulate the trans-activation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. Recombinant human CIAO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

40 kDa (362aa) confirmed by MALDI-TOF

Additionnal Information

CIAO1, Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39, ATGP2006-10 µg, ATGP2006-20 µg, ATGP2006-50 µg, ATGP2006-100 µg, ATGP2006-250 µg, ATGP2006-500 µg, ATGP2006-1 mg, ATGP2006-10, ATGP2006-20, ATGP2006-50, ATGP2006-100, ATGP2006-250, ATGP2006-500, ATGP2006-1

References & Citations

Johnstone RW, Wang J, et al. (1998) . J Biol Chem. 273 (18) :10880-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004795

Uniprot Accession Number

O76071

Species

Human

AA Sequence

MKDSLVL LGRVPAHPDS RCWFLAWNPA GTLLASCGGD RRIRIWGTEG DSWICKSVLS EGHQRTVRKV AWSPCGNYLA SASFDATTCI WKKNQDDFEC VTTLEGHENE VKSVAWAPSG NLLATCSRDK SVWVWEVDEE DEYECVSVLN SHTQDVKHVV WHPSQELLAS ASYDDTVKLY REEEDDWVCC ATLEGHESTV WSLAFDPSGQ RLASCSDDRT VRIWRQYLPG NEQGVACSGS DPSWKCICTL SGFHSRTIYD IAWCQLTGAL ATACGDDAIR VFQEDPNSDP QQPTFSLTAH LHQAHSQDVN CVAWNPKEPG LLASCSDDGE VAFWKYQRPE GL