Recombinant human Xg protein

XG is the XG blood group antigen, and is at the pseudoautosomal boundary on the short (p) arm of chromosome X. The three 5' exons reside in the pseudoautosomal region and the remaining exons within the X-specific end. A truncated copy of this gene is found on the Y chromosome at the pseudoautosomal boundary. It is transcribed, but not expected to make a Y-chromosome specific gene product. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Recombinant human XG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glycoprotein Xg isoform 1 precursor, PBDX

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

15.5 kDa (144aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Xg, Glycoprotein Xg isoform 1 precursor, PBDX, ATGP2005-10 µg, ATGP2005-20 µg, ATGP2005-50 µg, ATGP2005-100 µg, ATGP2005-250 µg, ATGP2005-500 µg, ATGP2005-1 mg, ATGP2005-10, ATGP2005-20, ATGP2005-50, ATGP2005-100, ATGP2005-250, ATGP2005-500, ATGP2005-1

References & Citations

Gandouz, R., et al. (2008) Transfus Clin Biol 15 (4), 174-176; ; Fouchet, C., et al. (2000) Immunogenetics 51 (8-9), 688-694;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_780778

Uniprot Accession Number

P55808

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSQRDFDLA DALDDPEPTK KPNSDIYPKP KPPYYPQPEN PDSGGNIYPR PKPRPQPQPG NSGNSGGYFN DVDRDDGRYP PRPRPRPPAG GGGGGYSSYG NSDNTHGGDH HSTYGNPEGN MVAK