Recombinant human GEMIN6 protein

GEMIN6 is part of a large macromolecular complex, localized to both the cytoplasm and the nucleus, that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs) . Other members of this complex includes SMN, GEMIN2, GEMIN3, GEMIN4, and GEMIN5. Recombinant human GEMIN6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Gem-associated protein 6, FLJ23459, Gemin-6, SIP2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.9 kDa (190aa) confirmed by MALDI-TOF

Additionnal Information

GEMIN6, Gem-associated protein 6, FLJ23459, Gemin-6, SIP2, ATGP2004-10 µg, ATGP2004-20 µg, ATGP2004-50 µg, ATGP2004-100 µg, ATGP2004-250 µg, ATGP2004-500 µg, ATGP2004-1 mg, ATGP2004-10, ATGP2004-20, ATGP2004-50, ATGP2004-100, ATGP2004-250, ATGP2004-500, ATGP2004-1

References & Citations

Carissimi, C., et al. (2006) J. Biol. Chem. 281 (48), 37009-37016; ; Pellizzoni, L., et al. (2002) J. Biol. Chem. 277 (9), 7540-7545;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_079051

Uniprot Accession Number

Q8WXD5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSEWMKK GPLEWQDYIY KEVRVTASEK NEYKGWVLTT DPVSANIVLV NFLEDGSMSV TGIMGHAVQT VETMNEGDHR VREKLMHLFT SGDCKAYSPE DLEERKNSLK KWLEKNHIPI TEQGDAPRTL CVAGVLTIDP PYGPENCSSS NEIILSRVQD LIEGHLTASQ