Recombinant human PARP2 protein

PARP2 is poly (ADP-ribosyl) transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly (ADP-ribosyl) ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. Recombinant human PARP2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

42.5 kDa (376aa)

Additionnal Information

PARP2, Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2, ATGP2003-10 µg, ATGP2003-20 µg, ATGP2003-50 µg, ATGP2003-100 µg, ATGP2003-250 µg, ATGP2003-500 µg, ATGP2003-1 mg, ATGP2003-10, ATGP2003-20, ATGP2003-50, ATGP2003-100, ATGP2003-250, ATGP2003-500, ATGP2003-1

References & Citations

Schreiber V., et al. (2002) J. Biol. Chem. 277:23028-23036; ; Karlberg T., et al. (2010) Biochemistry. 49:1056-1058;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005475

Uniprot Accession Number

Q9UGN5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMQLDLR VQELIKLICN VQAMEEMMME MKYNTKKAPL GKLTVAQIKA GYQSLKKIED CIRAGQHGRA LMEACNEFYT RIPHDFGLRT PPLIRTQKEL SEKIQLLEAL GDIEIAIKLV KTELQSPEHP LDQHYRNLHC ALRPLDHESY EFKVISQYLQ STHAPTHSDY TMTLLDLFEV EKDGEKEAFR EDLHNRMLLW HGSRMSNWVG ILSHGLRIAP PEAPITGYMF GKGIYFADMS SKSANYCFAS RLKNTGLLLL SEVALGQCNE LLEANPKAEG LLQGKHSTKG LGKMAPSSAH FVTLNGSTVP LGPASDTGIL NPDGYTLNYN EYIVYNPNQV RMRYLLKVQF NFLQLW