Recombinant human Endothelin 2/EDN2 protein

EDN2 is a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This protein is involved in a wide range of biological processes, such as hypertension and ovulation. Recombinant human EDN2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Endothelin 2, ET2, PPET2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

19.9 kDa (177aa)

Additionnal Information

EDN2, Endothelin 2, ET2, PPET2, ATGP2001-10 µg, ATGP2001-20 µg, ATGP2001-50 µg, ATGP2001-100 µg, ATGP2001-250 µg, ATGP2001-500 µg, ATGP2001-1 mg, ATGP2001-10, ATGP2001-20, ATGP2001-50, ATGP2001-100, ATGP2001-250, ATGP2001-500, ATGP2001-1

References & Citations

Bourgeois C., et al. (1997) J. Clin. Endocrinol. Metab. 82:3116-3123; ; Ohkubo S., et al. (1990) FEBS Lett. 274:136-140;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001947

Uniprot Accession Number

P20800

Species

Human

AA Sequence

QAAATLE QPASSSHAQG THLRLRRCSC SSWLDKECVY FCHLDIIWVN TPEQTAPYGL GNPPRRRRRS LPRRCQCSSA RDPACATFCL RRPWTEAGAV PSRKSPADVF QTGKTGATTG ELLQRLRDIS TVKSLFAKRQ QEAMREPRST HSRWRKR