Recombinant human PIM1 protein

PIM1 belongs to the Ser/Thr protein kinase family, and PIM subfamily. This protein is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AuG (CuG) and downstream AuG codons. Recombinant human Pim1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Non-specific serine/threonine protein kinase, PIM

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.4 kDa (274aa) confirmed by MALDI-TOF

Additionnal Information

PIM1, Non-specific serine/threonine protein kinase, PIM, ATGP1997-10 µg, ATGP1997-20 µg, ATGP1997-50 µg, ATGP1997-100 µg, ATGP1997-250 µg, ATGP1997-500 µg, ATGP1997-1 mg, ATGP1997-10, ATGP1997-20, ATGP1997-50, ATGP1997-100, ATGP1997-250, ATGP1997-500, ATGP1997-1

References & Citations

Koike N., et al. (2000) FEBS Lett. 467:17-21; ; Ionov Y., et al. (2003) Anticancer Res. 23:167-178;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_002639.1

Uniprot Accession Number

P11309

Species

Human

AA Sequence

YQVGPLLGS GGFGSVYSGI RVSDNLPVAI KHVEKDRISD WGELPNGTRV PMEVVLLKKV SSGFSGVIRL LDWFERPDSF VLILERPEPV QDLFDFITER GALQEELARS FFWQVLEAVR HCHNCGVLHR DIKDENILID LNRGELKLID FGSGALLKDT VYTDFDGTRV YSPPEWIRYH RYHGRSAAVW SLGILLYDMV CGDIPFEHDE EIIRGQVFFR QRVSSECQHL IRWCLALRPS DRPTFEEIQN HPWM