Recombinant human beta-III Tubulin protein

Tubulin, beta 3 class III, also known as TuBB3, is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TuBB3 plays a critical role in proper axon guidance and mantainance. Recombinant human TuBB3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tubulin beta 3 class III, Tubulin, beta 3 class III, beta-4, CDCBM, CFEOM3A, TuBB4, TUBB3, FEOM3, fibrosis of extraocular muscles, congenital

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

52.8 kDa (473aa)

Additionnal Information

TuBB3, Tubulin beta 3 class III, Tubulin, beta 3 class III, beta-4, CDCBM, CFEOM3A, TuBB4, ATGP1996-10 µg, ATGP1996-20 µg, ATGP1996-50 µg, ATGP1996-100 µg, ATGP1996-250 µg, ATGP1996-500 µg, ATGP1996-1 mg, ATGP1996-10, ATGP1996-20, ATGP1996-50, ATGP1996-100, ATGP1996-250, ATGP1996-500, ATGP1996-1

References & Citations

Katsetos C.D., et al. (2003) J. Child Neurol. 18:851-866; ; Tischfield M.A., et al. (2010) Cell. 140:74-87;

Other References

Shao Q, et al. Uncoupling of UNC5C with Polymerized TUBB3 in Microtubules Mediates Netrin-1 Repulsion. (J Neurosci. 2017) {https://pubmed.ncbi.nlm.nih.gov/28483977/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,10% glycerol, 1mM DTT

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_006077

Uniprot Accession Number

Q13509

Species

Human

AA Sequence

MREIVHI QAGQCGNQIG AKFWEVISDE HGIDPSGNYV GDSDLQLERI SVYYNEASSH KYVPRAILVD LEPGTMDSVR SGAFGHLFRP DNFIFGQSGA GNNWAKGHYT EGAELVDSVL DVVRKECENC DCLQGFQLTH SLGGGTGSGM GTLLISKVRE EYPDRIMNTF SVVPSPKVSD TVVEPYNATL SIHQLVENTD ETYCIDNEAL YDICFRTLKL ATPTYGDLNH LVSATMSGVT TSLRFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTARG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVATVF RGRMSMKEVD EQMLAIQSKN SSYFVEWIPN NVKVAVCDIP PRGLKMSSTF IGNSTAIQEL FKRISEQFTA MFRRKAFLHW YTGEGMDEME FTEAESNMND LVSEYQQYQD ATAEEEGEMY EDDEEESEAQ GPK