Recombinant human SDR16C5 protein

Short chain dehydrogenase/reductase family 16C, member5, also known as SDR16C5, is active in both the oxidative and reductive directions. This protein oxidizes all-trans-retinol in all-trans-retinaldehyde. No activity was detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH. Recombinant human SDR16C5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Short chain dehydrogenase/reductase family 16C member5, Short chain dehydrogenase/reductase family 16C, member5, RDH2, RDH-E2, RDHE2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.3 kDa (261aa) confirmed by MALDI-TOF

Additionnal Information

SDR16C5, Short chain dehydrogenase/reductase family 16C member5, Short chain dehydrogenase/reductase family 16C, member5, RDH2, RDH-E2, RDHE2, ATGP1993-10 µg, ATGP1993-20 µg, ATGP1993-50 µg, ATGP1993-100 µg, ATGP1993-250 µg, ATGP1993-500 µg, ATGP1993-1 mg, ATGP1993-10, ATGP1993-20, ATGP1993-50, ATGP1993-100, ATGP1993-250, ATGP1993-500, ATGP1993-1

References & Citations

Matsuzaka Y., et al. (2002) Biochem. Biophys. Res. Commun. 297:1171-1180; ; Lee S.-A., et al. (2009) Chem. Biol. Interact. 178:182-187;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_620419

Uniprot Accession Number

Q8N3Y7

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSPKPRKNV AGEIVLITGA GSGLGRLLAL QFARLGSVLV LWDINKEGNE ETCKMAREAG ATRVHAYTCD CSQKEGVYRV ADQVKKEVGD VSILINNAGI VTGKKFLDCP DELMEKSFDV NFKAHLWTYK AFLPAMIAND HGHLVCISSS AGLSGVNGLA DYCASKFAAF GFAESVFVET FVQKQKGIKT TIVCPFFIKT GMFEGCTTGC PSLLPILEPK YAVEKIVEAI LQEKMYLYMP K