Recombinant human SDR16C5 protein
Short chain dehydrogenase/reductase family 16C, member5, also known as SDR16C5, is active in both the oxidative and reductive directions. This protein oxidizes all-trans-retinol in all-trans-retinaldehyde. No activity was detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH. Recombinant human SDR16C5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Short chain dehydrogenase/reductase family 16C member5, Short chain dehydrogenase/reductase family 16C, member5, RDH2, RDH-E2, RDHE2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
28.3 kDa (261aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Matsuzaka Y., et al. (2002) Biochem. Biophys. Res. Commun. 297:1171-1180; ; Lee S.-A., et al. (2009) Chem. Biol. Interact. 178:182-187;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_620419
Uniprot Accession Number
Q8N3Y7
Species
Human
AA Sequence
Frequently Asked Questions
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